Antagonistic antibody prevents toll-like receptor 2–driven lethal shock-like syndromes
J. Clin. Invest. Guangxun Meng, et al. 113:1473 doi:10.1172/JCI20762 [Go to this article.]

Figure 1
Application of mAb T2.5 for specific detection of TLR2. (A–D) Results of flow cytometry of HEK293 cells stably overexpressing Flag-tagged mTLR2 (A) or human TLR2 (B), as well as primary TLR2^–/– (C) and wild-type murine macrophages (D), by staining with mAb T2.5 (b line). Negative controls represent cells incubated with a mouse IgG-specific secondary antibody only (filled areas). For positive controls, Flag-specific (A and B) and mTLR2-specific (C and D) polyclonal antisera were used (thin line). (E) For immunoprecipitation with T2.5, lysates of HEK293 cells overexpressing murine or human TLR2, as well as of murine RAW264.7 macrophages, were applied as indicated. TLR2 precipitates were visualized by application of Flag-specific (HEK293) or mTLR2-specific (RAW264.7) polyclonal antisera. Flag-specific beads (αFlag) and protein G beads in the absence of antibodies (pG), as well as vector-transfected HEK293 cells, were used as controls. The size of TLR2 was 97 kDa.