Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway
J. Clin. Invest. Michael Nardi, et al. 113:973 doi:10.1172/JCI20726 [Go to this article.]

Figure 7
Effect of anti–GPIIIa49–66 and 12(S)-HETE on murine platelet fragmentation of (A) 12-LO^–/– and (B) 5-LO^–/– mice. Gel-filtered murine platelets were treated with control Ab (Ca), anti–GPIIIa49–66 (Ga), 12(S)-HETE, or its steroisomer, 12(R)-HETE (rH). Note absence of anti–GPIIIa49–66–induced platelet fragmentation with 12-LO^–/– mouse platelets and its presence with 5-LO^–/– mouse platelets. Note presence of 12(S)-HETE–induced platelet fragmentation with WT, 12-LO^–/–, and 5-LO^–/– platelets. n = 3.