Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway
J. Clin. Invest. Michael Nardi, et al. 113:973 doi:10.1172/JCI20726 [Go to this article.]

Figure 6
Effect of anti–GPIIIa49–66 and 12(S)-HETE on platelet count and fragmentation of WT, NADPH oxidase gp91^phox–/–, Rac-2^–/–, and 12-LO^–/– platelets. (A) Platelet count. Mice were treated with 25 μg of control or patient anti–GPIIIa49–66, and platelet counts were monitored at various time intervals (n = 5 for each group). (B) Fragmentation. Percentage of platelet particles were enumerated after in vitro exposure to anti–GPIIIa49–66 (25 μg/ml) (Ga), control IgG (Cg), or 12(S)-HETE (100 nM) (H) for 4 hours at 37°C.