Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway
J. Clin. Invest. Michael Nardi, et al. 113:973 doi:10.1172/JCI20726 [Go to this article.]

Figure 5
Effect of 12(S)-HETE on platelet oxidation. (A) Gel-filtered human platelets were preincubated with various concentrations of catalase and DPI for 15 minutes prior to the addition of 12(S)-HETE (100 nM) for 4 hours. Cb, control buffer; H, 12(S)-HETE with no drug. Increasing numbers refer to doubling concentrations of catalase, starting at 125 μg/ml, and DPI, starting at 10 μM. (B) Gel-filtered platelets were loaded with DCFH and incubated with 12(S)-HETE at various concentrations for 4 hours at 37°C. Oxidation was measured by DCFH fluorescence.