Complement-independent Ab-induced peroxide lysis of platelets requires 12-lipoxygenase and a platelet NADPH oxidase pathway
J. Clin. Invest. Michael Nardi, et al. 113:973 doi:10.1172/JCI20726 [Go to this article.]

Figure 1
Absence of granulocytes and presence of the NADPH oxidase component gp91^phox in gel-filtered platelets. (A) Fluorescence flow-cytometry comparison of a leukocyte/platelet preparation with gel-filtered platelets. Lower-left panel: forward scatter–side scatter plot of an enriched leukocyte/platelet preparation. G, granulocytes; L, lymphocytes; Pl, platelets, as designated by their known scatter pattern. Upper-left panel: similar plot of gel-filtered platelets. Lower-right panel: anti–CD11b-FITC Ab reactivity specific for granulocytes/monocytes. Upper-right panel: similar plot of gel-filtered platelets. Granulocyte-reactive anti–CD11b-FITC Ab was unreactive with gel-filtered platelets (<0.1%). FSH, forward scatter height; SSH, side scatter height. (B) Reactivity of anti–gp91 Ab with granulocytes and platelets. Upper panels: control mouse mAb, reactivity with platelets and granulocytes. Lower panels: monoclonal anti–gp91 fluorescence.