Absence of platelet-activating factor receptor protects mice from osteoporosis following ovariectomy
J. Clin. Invest. Hisako Hikiji, et al. 114:85 doi:10.1172/JCI20504 [
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Figure 8Effect of PAFR on osteoclastic resorption stimulated by IL-1β. (
A) Radiographic images of parietal bones cultured in the presence of IL-1β (0.3 ng/ml) and/or WEB 2086 (100 μM). Scale bar: 1.0 mm. (
B and
C) Assessments of osteoclastic bone resorption. The resorption levels of the cultured parietal bones were semiquantitatively scored as described in Methods, and scores for individual bone tissues are presented in each graph. Bars depict means of groups. IL-1β treatment greatly enhanced the bone resorption. Both WEB 2086 (
B) and PAFR deficiency (
C) significantly suppressed the stimulatory effect of IL-1β. *
P < 0.0001 vs. control;
#P < 0.005 vs. IL-1β–treated osteoclasts in (
B) or IL-1β–treated PAFR-WT osteoclasts in (
C).
