Defining the pathogenic involvement of desmoglein 4 in pemphigus and staphylococcal scalded skin syndrome
J. Clin. Invest. Takeshi Nagasaka, et al. 114:1484 doi:10.1172/JCI20480 [Go to this article.]

Figure 2
IP-IB analysis of recombinant Dsg4 proteins in pemphigus and normal sera. (A) The hDsg4-His, which was produced by baculovirus expression, was immunoprecipitated with sera from patients with PF, mucocutaneous PV (PV-MC), mucosal dominant PV (PV-M), or normal individuals (N). The results from 10 representative sera are shown. The anti–E-tag mAb (E) was used as the positive control. Most of the PF and PV-MC sera, but none of the PV-M or N sera, reacted with hDsg4-His. (B) hDsg4-His, which was produced by eukaryotic expression in CHO cells, was immunoprecipitated by PF and PV-MC sera, but not by PV-M or normal sera. (C) The calcium dependency of the Dsg4 reactivity was determined. Removal of calcium by dialysis abolished the reactivities of the PF and PV-MC sera against Dsg4, which indicates that these sera recognize calcium-dependent conformational epitopes on Dsg4. (D) The mDsg4-His produced by baculovirus expression was immunoprecipitated by PF, PV-MC, PV-M, and N sera. Similar to the findings with hDsg4, many of the PF and PV-MC sera, but none of the PV-M or N sera, reacted with the mDsg4.