Bacterial flagellin is a dominant antigen in Crohn disease
J. Clin. Invest. Michael J. Lodes, et al. 113:1296 doi:10.1172/JCI20295 [
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Figure 8Dose response and specificity of C3H/HeJBir CD4
+ CBir1-specific T cell line. T cell line CBir-1B1 proliferated specifically in response to recombinant flagellin protein CBir1. Antigens used in the assay include recombinant flagellins CBir1 (filled circles) and Fla-X (open circles); the 38-kDa antigen of
M. tuberculosis (p38 antigen; 38 kDa: filled triangles); lysate of
E. coli antigens (
E. coli; open triangles); protein antigens extracted from mouse food pellets (Food Ag; filled squares); and a lysate of the ModeK epithelial cell line, of C3H origin (epithelial: open squares). Several randomly expressed recombinant commensal bacterial antigens were also tested and were negative (including randomly cloned C3H/HeJ mouse cecal bacterial antigens 99 [rIB99] and 32 [rIB32]). T cells plus APCs only are indicated by a filled diamond.
