Bacterial flagellin is a dominant antigen in Crohn disease
J. Clin. Invest. Michael J. Lodes, et al. 113:1296 doi:10.1172/JCI20295 [Go to this article.]

Figure 2
Schematic of recombinant flagellin constructs with percent similarity to related flagellin B from the anaerobe B. fibrisolvens (GenBank accession number AAB82613). (A) Structure of B. fibrisolvens flagellin B showing conserved NH₂ and carboxy (CO₂H-conserved) regions and the hypervariable central domain. (B) Diagram of the full-length amino acid sequence of mouse cecal bacteria flagellins CBir1 and Fla-X, indicating the similarity of the three domains with the respective B. fibrisolvens domains. (C and D) Schematics of recombinant flagellin proteins and fragments for CBir1 (C) and Fla-X (D) expressed in E. coli and purified by six-histidine tag affinity to nickel-nitrilotriacetic acid columns.