A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA–induced IL-12 production and Th cell differentiation
J. Clin. Invest. Kenji Sugimoto, et al. 114:857 doi:10.1172/JCI20014 [
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Figure 7Increased Th1 response to
L. major infection in Cot/Tpl2
–/– mice. (
A) The footpad swelling of age-matched WT (filled circles,
n = 5) and Cot/Tpl2
–/– (open circles,
n = 5) mice infected with
L. major was monitored on a weekly basis. (
B) The number of
L. major amastigotes in the popliteal lymph nodes of infected WT (black bars,
n = 4) and Cot/Tpl2
–/– mice (white bars,
n = 4) was examined 6 weeks after infection (see Methods). (
C) CD4
+ T cells were isolated from the lymph nodes of
L. major–infected WT (black bars,
n = 4) or Cot/Tpl2
–/– mice (white bars,
n = 4) after 6 weeks of infection and cultured (2 × 10
5 per well in 200 μl of culture medium) with soluble
L. major antigen (30 μg/ml) for 3 days. Cytokine production in the culture supernatants was examined by each cytokine-specific ELISA. Data are expressed as mean of wells ± SD and are representative of 3 independent experiments. *
P < 0.05.
