A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA–induced IL-12 production and Th cell differentiation
J. Clin. Invest. Kenji Sugimoto, et al. 114:857 doi:10.1172/JCI20014 [Go to this article.]

Figure 6
Antigen-specific Th1 cell responses were enhanced in Cot/Tpl2^–/– mice. (A) Lymph node cells were collected from WT (filled circles, n = 4) or Cot/Tpl2^–/– mice (open circles, n = 4) on day 9 after immunization with OVA in CFA. Proliferation of antigen-specific T cells was analyzed by cell proliferation assay using cell culture supernatants in different concentrations of OVA for 72 hours. (B) CD4⁺ T cells from lymph nodes of WT (black bars, n = 4) or Cot/Tpl2^–/– mice (white bars, n = 4) immunized with OVA in CFA were cultured in different concentrations of OVA with APCs for 72 hours. Cytokines in supernatants of antigen-stimulated pooled cells were determined by specific ELISA. (C and D) Serum was collected from WT (filled circles, n = 5) and Cot/Tpl2^–/– mice (open circles, n = 5) before (day 0) and after (day 9) immunization with OVA plus CFA (C) or OVA plus alum (D). OVA-specific IgG2a, OVA-specific IgG1, and OVA-specific IgE were analyzed. The y axis indicates the OD value at 492 nm. (E) CD4⁺ T cells from lymph nodes of WT (black bars, n = 3) or Cot/Tpl2^–/– mice (white bars, n = 3) immunized with OVA plus LPS or OVA plus CpG-DNA were cultured in the presence of OVA (1 mg/ml) with APCs for 72 hours. Cytokines in supernatants of antigen-stimulated pooled cells were determined by specific ELISA. Data are expressed as mean of wells ± SD and are representative of 3 independent experiments. *P < 0.05.