A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA–induced IL-12 production and Th cell differentiation
J. Clin. Invest. Kenji Sugimoto, et al. 114:857 doi:10.1172/JCI20014 [Go to this article.]

Figure 5
Maturation and cytokine production of DCs. (A) On day 5, bone marrow–derived DCs from WT or Cot/Tpl2^–/– mice were left unstimulated (shaded histograms) or stimulated with 1 μg/ml LPS or 1 μM CpG-DNA (open histograms) for 24 hours. The DCs were subjected to MHC class II, CD40, and CD86 expression analyses by flow cytometry. (B) Immature WT (black bars) and Cot/Tpl2^–/– (white bars) DCs (positively selected using magnetized Ab for CD11c) were unstimulated or stimulated with 1 μg/ml LPS or 1 μM CpG-DNA for 24 hours. IL-12 p40 in culture supernatants was measured by ELISA. Data are expressed as mean of wells ± SD and are representative of 3 independent experiments. *P < 0.05.