Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury
J. Clin. Invest. Lixin Sun, et al. 113:1364 doi:10.1172/JCI20001 [
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Figure 7SCF-stimulated progenitor cell migration in vivo. (
A) Quantification of BrdU-labeled cells in the injected areas (1.5 mm2) and in the contralateral side of the brain. Significantly more BrdU-labeled cells were seen in the SCF-injected area than in the PBS-injected area or in the contralateral side of the SCF-injected brain (
P < 0.001;
n = 6). inj., injection side; contra., side contralateral to SCF injection. (
B) Representative images of BrdU staining in the cortical areas. (
C) Schematic diagram of the brain used to examine the response of NPSC to rmSCF is shown. The blue arrow indicates the intracerebral injection track (as described in Methods). (
D) Normal distribution of BrdU-positive cells. These cells were detected mainly in the SVZ prior to SCF injection and were also positive for phospho-histone H3 staining (p-H3). (
E and
F) Immunohistochemistry of SCF-injected brain with BrdU and phospho-histone H3 antibodies in LVZ (
E) and SCF-injected cortex (
F). (
G) Nestin expression of BrdU-positive cells in the SVZ. (
H) Three-dimensional digital image of the cells indicated by the arrowhead in
G is shown. Upper, main, and right panels show views of the
xz,
xy, and
yz planes, respectively. Lines represent coordinates in each plane.
x axis, 23.2 ∝m;
y axis, 23.2 ∝m;
z axis, 11 ∝m; optical section thickness, 1.1 ∝m. Scale bar (shown in
B):
B, 100 ∝m;
D, 64 ∝m;
E and
F, 32 ∝m;
G, 12 ∝m.
