Neuronally expressed stem cell factor induces neural stem cell migration to areas of brain injury
J. Clin. Invest. Lixin Sun, et al. 113:1364 doi:10.1172/JCI20001 [
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Figure 4Expression of c-kit in NSPCs in vitro and in vivo. (
A) Expression of
c-kit mRNA in human NSPCs (hNSPC) and mouse NSPCs (mNSPC), as measured by semiquantitative RT-PCR. (
B and
C) Immunofluorescence analysis for c-kit and nestin in mouse (
B) and human (
C) NSPCs. (
D and
E) Expression of c-kit in NSPCs in vivo. (
D) Double staining of mouse brain by c-kit and nestin revealed c-kit expression in nestin-positive cells (arrows) in the SVZ. (
E) Representative orthogonal images of colocalization of c-kit and nestin in vivo. Upper, main, and left panels show views of the
xz,
xy, and
yz planes, respectively. Lines represent coordinates in each plane.
x axis, 24.4 ∝m;
y axis, 24.4 ∝m;
z axis, 6.7 ∝m; optical section thickness, 0.02 ∝m. Scale bar (shown in
B):
B_
D, 20 ∝m.
