Bim regulation may determine hippocampal vulnerability after injurious seizures and in temporal lobe epilepsy
J. Clin. Invest. Sachiko Shinoda, et al. 113:1059 doi:10.1172/JCI19971 [Go to this article.]

Figure 1
Seizure-induced hippocampal injury is associated with Bim overexpression. (A) Representative photomicrographs showing histopathology at 24 hours, in which marked loss of hippocampal CA3 neurons and the appearance of TUNEL are observed (top panels), while the cortex is largely spared (bottom panels). Inset in top left panel: Schematic of the hippocampal area from which images were taken (box). Inset in top right panel: ×100 magnification of a TUNEL-stained CA3 cell. Scale bar: 75 μm. (B) Representative Western blot (n = 2 per lane) showing increased hippocampal Bim_EL levels following seizures in the rat. Molecular weight markers (kDa) are given on the left, and time is shown in hours after termination of seizures. Staurosporine-treated rat C6 glioma cell lysates express all three Bim isoforms and are included as a positive control (C6+). α-Tubulin is shown below to confirm equivalency of protein loading. (C) Graph showing quantification of changes in Bim_EL expression (OD units) after seizures. *P < 0.05, **P < 0.01 vs. control (con). Data are from four rats per group. (D) Representative Western blot (n = 2 per lane) from cortex, revealing that Bim levels do not change in this region following seizures. Immunoblots are representative of two or three independent experiments.