Central and peripheral actions of somatostatin on the growth hormone–IGF-I axis
J. Clin. Invest. Robert D. Murray, et al. 114:349 doi:10.1172/JCI19933 [Go to this article.]

Figure 1
Cultured isolated rat hepatocytes incubated with (A) GH (1–1,000 ng/ml) for 24 hours and (B) GH (500 ng/ml) or buffer for 0–24 hours. Cells were harvested for RNA and analyzed for IGF-I mRNA and β-actin by RPA. The IGF-I riboprobe corresponded to a 166-bp fragment of the mature IGF-I protein, and β-actin corresponded to a 125-bp band. Upper and lower panels: representative blots; middle panel: mean fold IGF-I mRNA induction of 3 individual studies. *P < 0.05 compared with control.