Distinct roles of Smad pathways and p38 pathways in cartilage-specific gene expression in synovial fibroblasts
J. Clin. Invest. Hiroaki Seto, et al. 113:718 doi:10.1172/JCI19899 [
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Figure 6Induction of Alizarin red staining and type X collagen in MKK6-transduced SFs in pellet cultures. (A–F) SFs infected with MKK6
CA virus alone (A, C, and E) or together with Smad1 virus (B, D, and F) were subjected to pellet culture. Cultures were fixed with 3.7% formaldehyde 3 weeks later, and then stained with Alcian blue (A and B), Alizarin red (C and D) or anti–type X collagen (E and F). Note the increased Alcian blue staining and the reduced Alizarin red activity and type X collagen immunoactivity with Smad1 coexpression. Scale bars:100 μm (A–D) and 50 μm (E and F).
