Distinct roles of Smad pathways and p38 pathways in cartilage-specific gene expression in synovial fibroblasts
J. Clin. Invest. Hiroaki Seto, et al. 113:718 doi:10.1172/JCI19899 [Go to this article.]

Figure 1
Modulation of intracellular signaling pathways by adenovirus vector–mediated gene transduction into SFs. (A) SFs at passage 3 were transduced with HA-tagged constitutively active ALK3, ALK5, and ALK6, and the expressed products were detected by immunoblotting after 2 days of viral infection. Expression of these genes was detected by immunoblotting with anti-HA and phospho-Smad1, -Smad 5, and Smad8 (p-Smad1/5/8) was observed in cells expressing ALK3^CA or ALK6^CA, and p-Smad2, in cells expressing ALK5^CA. (B) Expression of Smad1 and 6 in SFs was determined by anti-Flag. (C) Adenovirus vector–mediated ALK^CA or MKK6^CA expression specifically activated p38 pathways in SFs, as determined by Western blotting with anti–phospho-p38 (p-p38). The increased p38 phosphorylation induced by ALK3^CA or MKK6^CA overexpression was suppressed by the p38-selective inhibitor SB203580.