OspC facilitates Borrelia burgdorferi invasion of Ixodes scapularis salivary glands
J. Clin. Invest. Utpal Pal, et al. 113:220 doi:10.1172/JCI19894 [
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Figure 2Binding of OspC F(ab)
2 fragments to
B. burgdorferi N40. (
a) Binding of anti-OspC sera (black bars), normal rabbit sera (white bars), IgG, or F(ab)
2 by ELISA.
B. burgdorferi N40 lysates were immobilized onto microtiter wells (10 μg/ml) and probed with a 1:100 dilution of sera (OspC sera) or normal rabbit sera (NRS), or 8 μg/ml of purified IgGs (OspC IgG or NRS IgG) or 8 μg/ml of purified F(ab)
2 fragments (OspC-Fab or NRS-Fab). Binding was detected using anti–rabbit F(ab)
2 fragment–specific goat IgG conjugated to horseradish peroxidase. Data represent the OD
450 at 15 minutes (mean ± SEM,
n = 3; differences between values of wells treated with OspC or NRS Ab were at least
P < 0.001). (
b) OspC F(ab)
2 fragments directly bind to the surface of intact
B. burgdorferi. Unfixed
B. burgdorferi were immobilized onto glass slides and incubated in the presence of F(ab)
2 fragments prepared from normal rabbit sera (NRS-Fab) or anti-OspC sera (anti–OspC-Fab). Binding was detected using anti–rabbit F(ab)
2 fragment–specific goat IgG labeled with FITC. Images were obtained using a 40× objective lens on a Zeiss LSM 510 confocal microscope (
n = 3).
