Glycoprotein 130 regulates bone turnover and bone size by distinct downstream signaling pathways
J. Clin. Invest. Natalie A. Sims, et al. 113:379 doi:10.1172/JCI19872 [Go to this article.]

Figure 3
Increased osteoclast formation in male and female gp130^Y757F/Y757F mutant mice. Tibial trabecular OcS/BS was significantly increased in male (a) and female (b) F/F mice compared with WT and Δ/Δ mice. All values are mean ± SEM from a minimum of eight mice per group at 12–16 weeks of age. *P < 0.05; **P < 0.01; ***P < 0.001 vs. WT of the same sex. (c) Representative images of TRAP-stained primary spongiosa from WT and F/F mice. Note the large number of TRAP-positive osteoclasts (arrows) and the absence of trabecular bone in the F/F section. gp, growth plate; m, marrow; tb, trabecular bone. Scale bar (black), 200 μm. (d and e) In vitro osteoclastogenesis assays demonstrating increased TRAP-positive MNCs (TRAP⁺ MNCs/well) generated from RANKL-induced primary bone marrow cultures (d) and BMMP cultures (e) from WT and gp130 mutant mice. (f) Pit formation by osteoclasts generated from RANKL-induced primary bone marrow cultures from WT and gp130 mutant mice. All values are mean ± SEM of quadruplicate cultures from four separate mouse bone marrows of mixed sexes. *P < 0.05; **P < 0.01; ***P < 0.005 vs. WT culture.