Th2-predominant inflammation and blockade of IFN-γ signaling induce aneurysms in allografted aortas
J. Clin. Invest. Koichi Shimizu, et al. 114:300 doi:10.1172/JCI19855 [
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Figure 1Aortic allografts from WT or GRKO recipients were harvested 1 week after transplantation and were analyzed for cytokine expression by RNase protection assay (RPA) (
A and
B) and Western blot for IL-4 (
C). (
A) Gel image of RPA. (
B) Histogram of the optical densities from RPA data normalized to GAPDH and averaged for 6 or 7 grafts. (
C) Protein extracts (25 μg/lane) of the aortic grafts of WT or GRKO recipients analyzed by Western blot for IL-4.