Concordant morphologic and gene expression data show that a vaccine halts HER-2/neu preneoplastic lesions
J. Clin. Invest. Elena Quaglino, et al. 113:709 doi:10.1172/JCI19850 [Go to this article.]

Figure 2
Immune events induced by prime and boost vaccination. (A) Titer of total Ab’s to rp185^neu. (B) Titers of Ig isotypes anti-rp185^neu in BALB-neuT mice. Values in A and B are described as sbp (see Methods section). (C) Percentage of CD4⁺ and CD8⁺ cells producing IFN-γ in the Spc of 22-week-old vaccinated mice. Data were obtained in triplicate. *P = 0.0002; **P = 0.0073. (D–F) Ab to rp185^neu downregulates membrane rp185^neu. rp185^neu+ TUBO cells were incubated with the immune serum from wk22pb mice at 4°C (D) and at 37°C (E and F). Large cytoplasmic dots show rp185^neu internalization at 37°C. These data are combined from three separate experiments. Magnification: D and E, ×100; F, ×200.