Nonclassical CD1d-restricted NK T cells that produce IL-13 characterize an atypical Th2 response in ulcerative colitis
J. Clin. Invest. Ivan J. Fuss, et al. 113:1490 doi:10.1172/JCI19836 [Go to this article.]

Figure 5
Expression of invariant chain TCR in LPMC CD3⁺ subpopulations. (A) LPMCs isolated from control, involved CD and UC tissues, as well as a control NKT cell line (see Methods), were stained with various fluorochrome-labeled Ab’s (CyChrome-labeled anti-CD3, FITC-labeled anti-Vα24, and PE-labeled anti-Vβ11). The resultant cells were gated on CD3 and analyzed for the expression of Vα24 versus Vβ11. (B) LPMCs isolated from control, involved CD and UC tissues, as well as a control NKT cell line, were stained with FITC-labeled anti-CD3 and PE-labeled α-GalCer_loaded CD1d tetramer. The resultant cells were then analyzed for surface expression of CD3 versus α-GalCer_loaded tetramer. The dot plots in both A and B are representative of results from three experiments.