Mast cells are required for experimental oral allergen–induced diarrhea
J. Clin. Invest. Eric B. Brandt, et al. 112:1666 doi:10.1172/JCI19785 [
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Figure 5Mucosal mast cell responses in allergic diarrhea. (
a) A scatter plot comparing the average difference in gene expression measured on the murine U74Av2 GeneChip following ten saline or OVA challenges was generated using GeneSpring software. The three lines indicate a twofold increase or twofold decrease compared with the mean. Representative, highly increased mast cell–related genes following diarrhea are indicated. (
b) MMCP-1 plasma levels were measured by ELISA following saline or OVA challenges. Representative fields (original magnification, ×125) of saline-challenged (
c) or OVA-challenged (
d) jejunum sections illustrate chloroaces) indicated by arrows (original magnification, ×500). (
e) Mucosal mast cell levels in the jejunum, ileum, and colon following three saline or OVA challenges were assessed by morphometric analysis of chloroacetate esterase–stained cells (
n = 3–4 mice per group;
#P < 0.0001;
##P = 0.013; *
P = 0.032; **
P = 0.003). (
f) A kinetic analysis of mast cell recruitment to the jejunum of saline- or OVA-challenged mice (
#P < 0.0005).
