Mast cells are required for experimental oral allergen–induced diarrhea
J. Clin. Invest. Eric B. Brandt, et al. 112:1666 doi:10.1172/JCI19785 [
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Figure 4Role of eosinophils in allergic diarrhea. (
a) Blood eosinophil levels in IL-5 transgenic (Tg) mice, IL-5/eotaxin-1 double-deficient mice (IL-5/Eot KO), and WT BALB/c mice were assessed 72 hours after the third saline or OVA challenge (mean ± SEM;
n = 4–10 mice; *
P < 0.0001;
#P < 0.004; **
P = 0.0035) (
b) Representative jejunum section from OVA-challenged WT, IL-5 transgenic, and IL-5/eotaxin-1 double-deficient mice, immunostained with anti-MBP Ab as described in Methods (arrows indicate eosinophils; original magnification, ×125). (
c) Jejunum eosinophil levels were assessed by morphometric analysis in IL-5 transgenic mice, IL-5/eotaxin-1 double-deficient mice, and WT BALB/c mice (mean ± SEM;
n = 4 mice; *
P < 0.05;
#P = 0.005; **
P = 0.004). (
d) Diarrhea occurrence in IL-5 transgenic, IL-5/eotaxin-1 double-deficient, and WT control mice following saline or OVA challenges.
