Mast cells are required for experimental oral allergen–induced diarrhea
J. Clin. Invest. Eric B. Brandt, et al. 112:1666 doi:10.1172/JCI19785 [
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Figure 1A murine model of allergen-induced diarrhea. (
a) The time line illustrates that mice were sensitized with two OVA/alum intraperitoneal (i.p.) injections (vertical hatch mark) and subsequently treated with intragastric (i.g.) saline or OVA challenges every 2–3 days (vertical lines). (
b) Diarrhea occurrence was assessed 15–45 minutes after intragastric challenges with either saline or increasing doses of OVA (2–50 mg in 250 μl of saline). (
c) Diarrhea occurrence in BALB/c mice (
n = 16) was compared with C57Bl/6 mice (
n = 8) following ten intragastric OVA challenges (50 mg). (
d) Representative photograph of the cecum and colon of BALB/c mice 60 minutes after ten intragastric saline or OVA challenges (upper and lower two samples, respectively). (
e) Nonsensitized mice (
n = 8) or OVA/alum–sensitized mice (
n = 15) were treated with repeated doses of intragastric OVA (50 mg). Following five doses of OVA, sensitized mice were challenged with BSA (50 mg) as a control before resuming OVA challenges.
