Induction of potent antitumor immunity by in situ targeting of intratumoral DCs
J. Clin. Invest. Katsuyoshi Furumoto, et al. 113:774 doi:10.1172/JCI19762 [Go to this article.]

Figure 1
CCL20 expression in the tumor site attracts DCs into and throughout the tumor mass. Mice were inoculated with CT26 or B16 tumors transduced with CCL20 or a mock vector, and DC infiltration at the tumor site was analyzed 10–15 days later. (A) Serial frozen tumor sections were stained with anti-CD11c Ab or isotype IgG control and counterstained with hematoxylin (magnification, ×200). Frozen sections of mock-transduced (B) or CCL20-transduced B16 tumors (C) stained with anti-CD11c Ab are shown (magnification, ×200). Frozen sections of the core of a CCL20-transduced B16 tumor stained with CD11c Ab are shown at magnifications of ×400 (D) and ×600 (E). Cell suspensions of tumors (F) and tumor-draining LNs (G) were analyzed for the presence of MHC class II⁺ CD11c⁺ DCs by flow cytometry. The results shown are the mean of three separate experiments. *Statistical differences between CCL20 and mock experimental groups.