Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells
J. Clin. Invest. Robert E. Hunger, et al. 113:701 doi:10.1172/JCI19655 [Go to this article.]

Figure 2
Phenotype and restriction pattern of antigen-specific T cell clones. (A) T cell lines B2.1 and B2.11 were analyzed for surface expression of CD3, CD4, CD8, and TCRαβ. (B) Cells were cultured with irradiated LC-like DCs in the presence or absence of antigen (Ag; sonicated M. leprae) with or without blocking antibodies as indicated. T cells were pulsed with ³H-thymidine after 72 hours of culture. Cells were harvested, and ³H incorporation was measured by a scintillation counter. The values shown are the mean ± SEM of triplicate cultures and are representative of at least three independent experiments. Student’s t test was used to compare cpm values of T cells stimulated with antigen alone versus antigen plus neutralizing antibodies. **P < 0.005.