Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells
J. Clin. Invest. Robert E. Hunger, et al. 113:701 doi:10.1172/JCI19655 [
Go to this article.]
Figure 1Phenotype of cord blood–derived LC-like DCs and MD-DCs. (A) LC-like DCs and MD-DCs were stained with mAb’s specific for CD1a (solid line) and mouse IgG control antibody (dashed line). MFI is indicated for the positive cell population. (B) LC-like DCs and MD-DCs were double-stained with mAb’s specific for CD1a and langerin. The numbers indicate the percentage of cells in each quadrant. One representative experiment is shown from four independent donors. (C) LC-like DCs and MD-DCs were stained with numerous surface proteins. MFI was determined by flow cytometry. For each data point, cells from three different donors were analyzed (± SEM). The ratios of MFI from LC-like DCs to MFI from MD-DCs were calculated and shown on a log scale as indicated.
