Abundant progenitor cells in the adventitia contribute to atherosclerosis of vein grafts in ApoE-deficient mice
J. Clin. Invest. Yanhua Hu, et al. 113:1258 doi:10.1172/JCI19628 [Go to this article.]

Figure 4
Immunostaining for Sca-1⁺ cells isolated with FACS sorting. Primary cultured adventitial cells were labeled with anti_Sca-1 antibody. Positive stained cells were sorted by FACS. (A) A single peak of isolated Sca-1⁺ cells (filled graph), showing no overlap with a negative control with only secondary antibody (green). (B_D) Microscopic photographs showing that the isolated cells were smeared, immunostained, and counterstained for nuclei. Note a result similar to that shown in Figure 3.