Abundant progenitor cells in the adventitia contribute to atherosclerosis of vein grafts in ApoE-deficient mice
J. Clin. Invest. Yanhua Hu, et al. 113:1258 doi:10.1172/JCI19628 [
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Figure 3Immunostaining for Sca-1
+ cells isolated with microbeads. Primary cultured adventitial cells were isolated with microbeads coupled with anti_Sca-1 antibody. The isolated cells were smeared, double immunostained for Sca-1 and Lin (
A_
C) or Sca-1 and c-kit (
D_
F), and counterstained for nuclei. Note that more than 90% of cells are Sca-1
+Lin
_c-kit
+.