Expression of the α1β1 integrin, VLA-1, marks a distinct subset of human CD4⁺ memory T cells
J. Clin. Invest. Itamar Goldstein, et al. 112:1444 doi:10.1172/JCI19607 [Go to this article.]

Figure 5
The VLA-1⁺ CD4⁺ T cells have a distinct cell surface phenotype. (a) Fresh CD4⁺ PBLs were analyzed for the cell surface coexpression of CD45RA and VLA-1, and the four quadrants were identified as R1 to R4, where R1 = VLA-1⁺ CD45RA^–; R2 = VLA-1^– CD45RA^–; R3 = VLA-1^– CD45RA⁺; and R4 = VLA-1⁺ CD45RA⁺. (b) Isolated CD4⁺ T cells were further purified into VLA-1⁺ and VLA-1^– fractions and then analyzed for the coexpression of CD45RA together with CCR7, CD62L, or VLA-4. The histograms represent the viable CD4⁺ T cell gate, and the data are representative of more than five PBL samples from different normal individuals. MFI, mean fluorescence intensity.