Expression of the α1β1 integrin, VLA-1, marks a distinct subset of human CD4⁺ memory T cells
J. Clin. Invest. Itamar Goldstein, et al. 112:1444 doi:10.1172/JCI19607 [Go to this article.]

Figure 4
VLA-1 expression is associated with Th1 polarization. (a) CD4⁺ PBLs were purified into VLA-1⁺ and VLA-1^– fractions, activated with PMA/ionomycin for 5 hours, and analyzed for intracellular IFN-γ production. (b) CD4⁺ PBLs were stimulated with TT or anti-CD3; 10 days later the cells were harvested and purified into VLA-1⁺ and VLA-1^– fractions, activated with PMA/ionomycin, and analyzed for intracellular IFN-γ and IL-4. (c) CD4⁺ PBLs were labeled with CFSE, stimulated with TT, and 10 days later purified into VLA-1⁺ and VLA-1^– fractions. Subsequently, the two subsets were activated and analyzed for divisions and intracellular IFN-γ. (d) CD4⁺ PBLs were stimulated with TSST-1 in two different environments: Th1 (IL-12⁺) or TH2 (IL-4⁺ and anti–IFN-γ). Ten days later cells were harvested and analyzed for VLA-1 and Vβ2 surface expression (left) or activated and analyzed for intracellular IFN-γ (right). (e) Fresh SFLs from RA (n = 4) and PsA (n = 2) patients were immediately activated and analyzed for surface expression of CD4, VLA-1, and intracellular IFN-γ. The dot plots were obtained by pregating on CD4⁺ events, and a representative patient sample is shown.