Expression of the α1β1 integrin, VLA-1, marks a distinct subset of human CD4⁺ memory T cells
J. Clin. Invest. Itamar Goldstein, et al. 112:1444 doi:10.1172/JCI19607 [Go to this article.]

Figure 2
VLA-1 expression preferentially distinguishes a large fraction of the CD4⁺ T cells responding to recall antigens. (a) CD4⁺ T cells from PBLs were labeled with CFSE and plated in 24-well plates at 10⁶ cells/well containing 2 × 10⁶ irradiated APCs and triggered by TT (upper panels), TSST-1 (middle panels), or medium (lower panels). The cells were harvested on day 8 and analyzed for CFSE content and the expression of VLA-1, CD25, or CD45RO. The data shown are representative of more than ten independent experiments in different donors. (b) CD4⁺ PBLs isolated from a PPD⁺ and TT-vaccinated individual were labeled with CFSE and triggered with TT, PPD, or TSST-1, harvested on day 8, and analyzed for generation number and VLA-1 expression. (c) CD4⁺ T cells from fresh PBLs were purified into VLA-1⁺ and VLA-1^– fractions and analyzed for relative size (FSC/SSC) and coexpression of CD25 and CD69. The data are representative of more than five different blood donors.