Expression of the α1β1 integrin, VLA-1, marks a distinct subset of human CD4⁺ memory T cells
J. Clin. Invest. Itamar Goldstein, et al. 112:1444 doi:10.1172/JCI19607 [Go to this article.]

Figure 1
VLA-1 is expressed only in a subset of the activated CD45RO⁺ CD4⁺ T cells. (a) CD4⁺ T cells were isolated from PBLs as described in Methods. Subsequently, the cells were stimulated with irradiated APCs and TSST-1 (upper four panels) or anti-CD3 (bottom panel). The cultures were assayed at day 8 for the cell surface coexpression of CD4 and Vβ2, together with VLA-1, CD45RO, CD25, or VLA-2. The data shown are representative of at least ten independent experiments in different normal donors. (b) CD4⁺ PBLs were stimulated twice (every 10 days) with APCs and TSST-1. Subsequently, the cultures were purified into VLA-1^– or VLA-1⁺ cell fractions. The cells were maintained in recombinant human IL-2–containing medium and stimulated every 10 days. The two cultures were analyzed for surface expression of VLA-1, both at day 1 and 8 weeks later. The data are representative of more than five independent experiments in different normal blood donors.