Stat-3 is required for pulmonary homeostasis during hyperoxia
J. Clin. Invest. Isamu Hokuto, et al. 113:28 doi:10.1172/JCI19491 [Go to this article.]

Figure 2
STAT-3 immunostaining in the Stat-3^Δ/Δ mice. Dams were treated with doxycycline from E0. Lung sections were prepared at E18.5 (a–d) or at 6 weeks of age (e–h). Tissues were stained with H&E (a, c, e, g) or immunostained for STAT-3 (b, d, f, h). Lung morphology was not altered in the Stat-3^Δ/Δ mice. Complete or nearly complete loss of STAT-3 staining was observed in respiratory epithelial cells at E18. Likewise, staining was observed in alveolar type II and conducting airway cells at PN 6 weeks in Stat-3^Δ/Δ mice (arrows). STAT-3 was detected in alveolar macrophages and in other nonepithelial cells in control and Stat-3^Δ/Δ mice (f and h) (arrowheads). Figures are representative of n ≥ 3 per genotype. Scale bar: 100 μm (a, c, e, g) or 50 μm (b, d, f, h).