Stat-3 is required for pulmonary homeostasis during hyperoxia
J. Clin. Invest. Isamu Hokuto, et al. 113:28 doi:10.1172/JCI19491 [
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Figure 10Exogenous SP-B protects
Stat-3Δ/Δ mice during hyperoxia. (
a) Lung morphology of the
Stat-3Δ/Δ mice treated with SP-B/DPPC/POPG (
a and
b) or DPPC/POPG (
c and
d) during hyperoxia exposure is shown. Lungs were excised 65 hours after exposure to 95% oxygen and stained with H&E. The lungs of SP-B/DPPC/DOPG–treated mice had less inflammatory cell infiltration, and epithelial cell necrosis was not observed (
a and
b). Alveolar thickening, inflammation, and epithelial cell necrosis was widespread in mice treated with DPPC/DOPG alone (
c and
d). Photomicrographs are representative of
n = 5 from each group. Bars = 100 μm. (
e) SP-B enhances survival of
Stat-3Δ/Δ mice during hyperoxia.
Stat-3Δ/Δ mice were placed in 95% oxygen and treated (intratracheally) with SP-B/DPPC/DOPG or DPPC/DOPG as described in Methods. Survival on day 4 was significantly increased in SP-B/DPPC/DOPG–treated mice; *
P < 0.05. On day 5, more SP-B/DPPC/DOPG–treated mice survived, but differences were not statistically significant;
n = 8 per group.
