Activation of antigen-presenting cells by microbial products breaks self tolerance and induces autoimmune disease
J. Clin. Invest. Hanspeter Waldner, et al. 113:990 doi:10.1172/JCI19388 [Go to this article.]

Figure 4
CpG ODN induces expansion of APC subsets and upregulation of MHC class II and CD86. Spleen cells from B10.S mice were stimulated with CpG ODN or non–CpG ODN. Spleen cells were subsequently stained with FITC-conjugated anti-CD11b, anti-CD11c, and anti-CD19 and PE-conjugated anti-CD86 or biotinylated anti–I-A^q/s. Expression of CD86 and MHC class II was determined on CD11c⁺-, CD11b⁺-, or CD19⁺-gated cells. Dotted lines indicate isotype controls. Numbers refer to mean of fluorescence intensity of specific staining (upper number) shown on gated APC subsets (%).