Activation of antigen-presenting cells by microbial products breaks self tolerance and induces autoimmune disease
J. Clin. Invest. Hanspeter Waldner, et al. 113:990 doi:10.1172/JCI19388 [Go to this article.]

Figure 3
Activation/maturation state and T cell-activating capacity of APCs. (A–E) Spleen cells (A–C) or BM-derived APCs (D and E) from wild-type B10.S and SJL mice were stained with PE-conjugated antibodies against the indicated subsets of APCs and biotinylated anti–I-A^q/s. The level of MHC class II expression was determined on gated APC subsets as indicated (%) (A and D) by flow cytometry and is shown as mean of fluorescence intensity (MFI) (B, C, and E). Data from one of three experiments with similar data are shown. (F) The indicated numbers of irradiated spleen cells from wild-type SJL or B10.S mice as APCs in presence of PLP139–151 were incubated with 5B6 transgenic SJL T cells and were subsequently pulsed with [³H]thymidine. T cell proliferation was measured by [³H]thymidine incorporation assay. Values are shown as mean cpm ± SD of triplicate wells. One experiment representative of three is shown.