Acquired predisposition to mycobacterial disease due to autoantibodies to IFN-γ
J. Clin. Invest. Beate Kampmann, et al. 115:2480 doi:10.1172/JCI19316 [
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Figure 3Recovery of IFN-γ from patient or control serum. Increasing concentrations of purified IFN-γ were added to patient (red) or control serum (blue). After 1 hour incubation at room temperature, the concentration of IFN-γ in the serum was determined by ELISA. Exogenous IFN-γ was not recovered from the patients’ serum even when added at concentrations 5 logs greater than that required for recovery from normal serum. The figure shows a representative experiment using serum from patient 1. All samples were set up in triplicate and supernatants pooled for IFN-γ ELISA measurements in duplicate wells. The CVs between duplicates were less than 5%.
