Acquired predisposition to mycobacterial disease due to autoantibodies to IFN-γ
J. Clin. Invest. Beate Kampmann, et al. 115:2480 doi:10.1172/JCI19316 [
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Figure 2IFN-γ–induced TNF-α upregulation is inhibited in the presence of patients’ sera. PBMCs from healthy donors showed marked upregulation of TNF-α production when exposed to concentrations of IFN-γ as low as 20 ng/ml in the presence of control serum (black) or fetal calf serum (blue). Addition of IFN-γ failed to upregulate TNF-α production in the presence of patients’ serum (red) at concentrations of IFN-γ from 20 to 100 ng/ml, and upregulation was only seen at very high concentrations. The figure shows a representative experiment using serum from patient 1. All samples were set up in triplicate and supernatants pooled for IFN-γ ELISA measurements in duplicate wells. The CV between duplicates was less than 5%.
