Acquired predisposition to mycobacterial disease due to autoantibodies to IFN-γ
J. Clin. Invest. Beate Kampmann, et al. 115:2480 doi:10.1172/JCI19316 [
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Figure 1IFN-γ production in response to mitogen stimulation in whole blood (WB) and isolated PBMCs. IFN-γ was not detected in patients’ plasma derived from whole blood after stimulation with PHA (or PMA/ionomycin in patient 2), whereas large quantities of IFN-γ were detected when isolated PBMCs were studied in the absence of patient plasma. IFN-γ production in PBMCs is expressed as a percentage of that in blood of healthy controls studied in the same experiments. Supernatants from triplicate samples of whole blood or PBMCs were pooled for each patient and control and assayed in duplicate by ELISA. The coefficients of variation (CVs) in each assay were less than 5%.
