Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia
J. Clin. Invest. Guang Yang, et al. 114:669 doi:10.1172/JCI19300 [Go to this article.]

Figure 2
Visualization of NF-κB activation in NF-κB/luc Tg mice. (A) In the pseudoimages, the blue to red coloring represents the lowest to the highest light intensity. 0–72 hours, duration of hyperoxia. (B) Immunohistochemical staining of luciferase protein in the neonatal lung after 24 hours of hyperoxia. Luciferase protein was localized to bronchiolar and alveolar epithelial cells (lower left and middle panels, white arrows) as well as endothelial cells (lower left panel, arrowhead). Colocalization of the luciferase (red) and alveolar macrophage CD68 (green) is demonstrated by yellow-colored cells (lower right panel, yellow arrow). Note that the luciferase protein is not exclusively localized to the macrophages.