Central role of mitochondrial aldehyde dehydrogenase and reactive oxygen species in nitroglycerin tolerance and cross-tolerance
J. Clin. Invest. Karsten Sydow, et al. 113:482 doi:10.1172/JCI19267 [
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Figure 4Effects of in vivo GTN treatment on GTN metabolism as assessed by the conversion of
14C-labeled GTN into 1,2-GDN. The enzymatic metabolite is significantly decreased in vessels from tolerant animals. Three rat thoracic aortas were cut into small pieces and incubated with 1 μM
14C-labeled GTN for 2 minutes at 37°C (Krebs buffer). GTN and its metabolites were separated and quantified as described in Methods. Data are mean ± SEM from six separate experiments. *
P < 0.05 vs. control.
