Obesity is associated with macrophage accumulation in adipose tissue
J. Clin. Invest. Stuart P. Weisberg, et al. 112:1796 doi:10.1172/JCI19246 [
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Figure 8Adipose tissue macrophages express proinflammatory factors. Perigonadal adipose tissue was collected from female B6.V
Lepob/ob mice, digested, and centrifuged to yield a buoyant adipocyte-enriched cell population (gray bars) and a pellet of SVCs. The SVCs were separated into F4/80
+ macrophages (black bars) and F4/80– populations (white bars) via FACS. Quantitative RT-PCR was used to measure the relative expression of three proinflammatory genes (
Tnfa,
Nos2, and
Il6). Expression of
Tnfa was limited almost exclusively to adipose tissue macrophages. F4/80– SVCs did not express detectable amounts of
F4/80 or
Tnfa (as indicated by asterisks). The adipocyte-enriched fractions expressed
Tnfa at levels commensurate
F4/80 expression and consistent with macrophage contamination.
Nos2 was expressed by both macrophages and F4/80– SVCs, and
Il6 was detectably expressed by all three populations in adipose tissue.
