Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity
J. Clin. Invest. Lesley E. Smythies, et al. 115:66 doi:10.1172/JCI19229 [Go to this article.]

Figure 9
Detection of TGF-β. (A–D) TGF-β was detected (arrows) in (A) lamina propria cells that stained with (B) mast cell marker c-kit but not (C) macrophage marker HAM56 and in (D) epithelial cells (magnification, ×40). (E) Soluble TGF-β protein was detected in S-CM but not E-CM or MNL-CM (each normalized to 2 mg/ml total protein), which were generated from lamina propria stroma, intestinal epithelial cells, and intestinal mononuclear cells, respectively (mean values for CMs from 3 separate donors). (F) TGF-β mRNA was present in whole tissue, stroma and epithelial cells, but not lamina propria (L.P.) macrophages from a representative donor (n = 3). Insets show a TGF-β⁺ (A), c-kit⁺ (B), and HAM56^– (C) cell at high magnification (×100).