Human intestinal macrophages display profound inflammatory anergy despite avid phagocytic and bacteriocidal activity
J. Clin. Invest. Lesley E. Smythies, et al. 115:66 doi:10.1172/JCI19229 [Go to this article.]

Figure 4
S-CM downregulation of monocyte surface antigen expression. (A) Blood monocytes cultured for 4 days in the absence or presence of S-CM, E-CM, or MNL-CM at the indicated concentrations were analyzed by FACS for HLA-DR, CD13, CD14, and CD16 (open histograms). Cells were also stained with FITC IgG1 and PE IgG2a irrelevant Abs (solid histograms). FACS insets show the flow-cytometric analysis of intestinal macrophages for the same surface marker. Data are from a representative experiment (n = 3). Electron micrograph inset shows a representative monocyte after 24-hour culture with S-CM (150 μg/ml total protein). (B) Blood monocytes incubated with S-CM (150 μg/ml total protein) and in the absence or presence of protease inhibitors, including trypsin, chymotrypsin, pronase, thermolysin, papain, and pancreas extract, as described in Methods, were analyzed by FACS as above.