CII-DC-AdTRAIL cell gene therapy inhibits infiltration of CII-reactive T cells and CII-induced arthritis
J. Clin. Invest. Zhongyu Liu, et al. 112:1332 doi:10.1172/JCI19209 [Go to this article.]

Figure 6
DCs migrate to the spleen. DCs were isolated from the bone marrow of DBA/1j mice and cultured in the presence of GM-CSF for 4 days. They were then transfected with AdGFP at 50 pfu/cell for 18 hours in vitro before being intraperitoneally injected into CII-primed DBA/1j mice. Forty-eight hours later, the spleen was collected and embedded with OCT. Ten-micrometer frozen sections were counterstained with Hoechst and examined by fluorescence microscope, then photographed. Original magnification, ×40. (a) The spleen section from DBA/1j mice injected with DC-AdGFP. (b) The spleen section from DBA/1j mice injected with PBS. DC-AdTRAIL+DOX induces activated T cell apoptosis in the spleen. The DBA/1j mice were treated with either DC-AdTRAIL+DOX or DC-AdGFP+DOX, as described above. The spleen was then collected and embedded with paraffin. In situ TUNEL staining was performed. Original magnification, ×40. (c) The spleen section from the mice treated with DC-AdTRAIL+DOX. (d) The spleen section from the mice treated with DC-AdGFP+DOX.