CII-DC-AdTRAIL cell gene therapy inhibits infiltration of CII-reactive T cells and CII-induced arthritis
J. Clin. Invest. Zhongyu Liu, et al. 112:1332 doi:10.1172/JCI19209 [Go to this article.]

Figure 5
CII-pulsed DCs are required for elimination of CII-reactive T cells. (a) Spleen T cells were isolated from the mice of various treatment groups shown at the bottom of the figure at the time of sacrifice (19 weeks of age) and were stimulated with γ-irradiated syngeneic spleen cells from DBA/1j mice that were pulsed with CII for 72 hours. The CII-specific T cell–proliferative response was measured using a ³H-thymic uptake assay. The proliferation of the CII-specific T cells was determined after an 18-hour pulse of ³H-thymidine. The counts were determined using a scintillation counter. There was a statistically significant decrease of T cell proliferation in the CII-DC-AdTRAIL+DOX–treatment group, compared with the other treatment groups. **P < 0.01. (b) Decreased secretion of IFN-γ in the CII-DC-AdTRAIL+DOX–treatment group. IFN-γ was determined in the supernatant at 72 hours after culture by an ELISA assay. The results represent the mean plus or minus SEM of duplicate cultures of ten mice per group analyzed separately. There was a statistically significant decrease of IFN-γ production in the CII-DC-AdTRAIL+DOX treatment group compared with the CIA–no treatment group and other treatment groups. **P < 0.01.