CII-DC-AdTRAIL cell gene therapy inhibits infiltration of CII-reactive T cells and CII-induced arthritis
J. Clin. Invest. Zhongyu Liu, et al. 112:1332 doi:10.1172/JCI19209 [Go to this article.]

Figure 4
CII-DC-AdTRAIL+DOX treatment inhibits T cell infiltration. (a) In situ staining of CD3⁺ T cells in the joint area. DBA/1j mice were immunized with CII and treated with CII-DC-AdTRAIL+DOX, CII-DC-AdTRAIL, CII-DC-AdGFP+DOX, or DC-AdTRAIL+DOX. The mice were sacrificed at 19 weeks of age, and the joints were sectioned and stained with anti-CD3 Ab. Dark brown cells indicate CD3-positive T cells. C, cartilage; BM, bone marrow cavity; SH, synovial hyperplasia. Original magnification, ×40. (b) Quantitative analysis of T cell infiltration. The numbers of CD3 positive T cell infiltrating in the joint area were determined blindly as described in Methods. At least five areas from each specimen were chosen randomly to determine the numbers of T cell infiltrating in each specimen. T cell infiltration of the synovial area of CII-DC-AdTRAIL+DOX–treated mice was significantly reduced, compared with the CIA–no treatment group or other treatment groups. *P < 0.05; **P < 0.01.